The qPCR data statistical analysis
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چکیده
Since the invention of real-time PCR (qPCR), thousands of high-impact studies have been conducted and published using qPCR technique (Heid et al. 1996; Higuchi et al. 1993; VanGuilder, Vrana, and Freeman 2008). Because it is highly sensitive, qPCR is the preferred method for microarray data validation (Canales et al. 2006); however the most exciting applications have been in the discovery of new biomarkers and in diagnostic prediction (Gillis et al. 2007). Despite the fact that this technique has been widely used by researchers, there are several obstacles to analyzing the vast amounts of data generated.
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تاریخ انتشار 2009